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KMID : 0366919930050010030
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Sungkyun Pharmceutical Journal
1993 Volume.5 No. 1 p.30 ~ p.33
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High Performance Liquid Chromatographic Analysis of Omeprazole in Human Plasma
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Kam, Sung-Hoon/감성훈
Lee, Yoo-Chul/Lee, Jang-Won/Chi, Sang-Cheol/이유철/이장원/지상철
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Abstract
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An HPLC procedure with UV detection has been developed fro the quantitation of omeprazole in human plasma. The drug and the intermal standard (benzocaine) were extracted from plasma with ethyl acetate and quantitated using a reverse-phase C_18 column. The chromatograms were completely free from interfering peaks, and the retention time of the omeprazole and the internal standard were 6.5 min and 10.0 min, respectively. Calibration plots were linear over the concentration range of 100∼1,000 ng/ml of omeprazole with the correlation coefficients all higher than 0.999. The mean interday precision and accuracy over three days were 3.12% and 4.02%, respectively. The mean interday precision and accuracy over three days were 3.12% and 4.02%, respectively. The mean recoveries over the entire calibration range were 96.2% for the drug and 91.3% for the internal standard. The limit of detection for omeprazole was 0.5 ng/ml. The method was simple, reliable and accurate for the quantitation of omeprazole in human plasma.
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KEYWORD
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